Myco testing

Mycoplasma testing (Danielle)

Use Mycoalert kit

Sample prep: Place 100uL media into labeled 1.5mL eppendorf. Test immediately or store on ice for a few hours or O/N at -20C.

Myco test:
 * Take out and thaw appropriate amount of "R" and "S" (50uL each per sample)
 * Turn on luminometer in cryo room
 * Add 50uL “R” to 100uL sample
 * Wait 5 mins
 * Set settings on luminometer: delay 0, integral 4, replicates 1, set to STD, sensitivity 50%
 * Set sample in, hit “read”
 * Record value “A”
 * Add 50uL “S” and wait 10mins
 * Go read again
 * If second number is lower than first, sample is negative (yay)….otherwise, if positive, it will be WAY bigger than first number
 * For borderline samples, allow media to sit on them for another day and then re-test
 * Media should not be changed the day before myco testing