RNA gel

Gel: 50mL 1% agarose 1% MOPs gel with formaldehyde


 * 0.5g agarose
 * 50mL 1X MOPS buffer
 * Boil together 2 minutes
 * Add 2.5mL formaldehyde per 50mL gel
 * pour into small gel mold, cool

RNA samples:

Run on gel @ 100V (for RNA quality, run ~2cm). Running buffer = 1X MOPS
 * combine RNA and 2X RNA buffer 1:1 in 1.5mL eppendorf
 * 65C for 5 mins
 * spin down
 * ice for 5 mins