Home-made ECL

Homemade ECL (From Ang/BZ)

Stock solutions:


 * Luminol (250mM)


 * P-coumaric acid (90mM)


 * 30% H2O2


 * 1M tris pH 8.5

ECL solutions

Solution A


 * 2mL 250mM luminol


 * 880uL 90mM p-coumaric acid


 * 20mL 1M tris pH8.5


 * 177mL ddH2O

Solution B


 * 120uL 30% H2O2


 * 20mL 1M tris pH 8.5


 * 180 mM ddH2O

Keep at 4C. To develop film, mix 1:1 and use immediately. (fades quickly)