H&E staining

From Katie

Fixing:

 * 10 sec 100% Methanol
 * 10 sec wash with tap water

Stain:

 * 20 min in hematoxylin
 * 10 sec wash with tap water
 * 1 min in bluing solution [0.25% ammonia hydroxygen or 0.5% Lithium hydroxygen]
 * 10 sec wash with tap water
 * 10 sec in 100% Ethanol
 * 2 min in Eosin
 * 10 sec wash in 100% EtOH
 * 10 sec wash in 100% EtOH
 * Dry
 * Add 2-3 drops Histo-clear
 * Add coverslip

Reagents:

 * Hematoxylin: Cat #7211, Richard-Allam Scientific 1pint/475ml
 * Eosin: Cat#7111, Richard-Allam Scientific
 * Histo-Clear: Cat#HS-200, Pational Diagnosis