DNAse treatment

Thaw RNA (can help to resuspend RNA by warming at 65C for 5 mins), spin down RNA Nanodrop/dilute to 200ng/uL Thaw DNAse deactivation beads at RT Master mix of enzyme, buffer, water; final reaction vol 15uL
 * 1.5uL 10X buffer
 * 0.75uL DNAse enzyme
 * 2.75uL H2O

Add 5uL master to tube, mix with 10uL 200ng/uL RNA 30 mins at 37C (water bath next to sink) 3uL (1/5vol) THAWED DNAse deactivation beads Cycle of flicking for 3 mins… Spin down, transfer sup to new tube (DO NOT BRING DNAse INACTIVATION BEADS ALONG WITH SUP--they inhibit the RT reaction/subsequent qPCR) Freeze at -80C or continue on to RNA check gel/cDNA synthesis