FBS aliquots

heat inactivation destroys heat labile complement proteins


 * Get 2 bottles of FBS from locked freezer (refrigerator key)
 * Thaw overnight at 4C (or in 37, but leave ice chunk in middle)—37 thaw takes ~ 1hr, go and mix it up on occasion
 * Head deactivate in waterbath : 56 degrees for 30 mins… invert to mix every 10 mins
 * Aliquot into 22 50mL conical labeled as FBS + date
 * Get dry ice container
 * Fill part way with dry ice
 * Spray FBS bottles with EtOH
 * Flame tops
 * Have all tubes labeled and with caps unscrewed (FBS+ DATE)
 * Pour 47.5ish mLs into each tube
 * Tighten caps
 * Sit tubes in dry ice (cover to top) for about an hour, put in freezer